Juan P. Advis

Juan P. Advis.

Bartlett Hall 213
Phone: 848-932-9240
Email: advis@sebs.rutgers.edu


To assess if the hypothalamic median eminence (ME) is a neuroendocrine control site where final hypophysiotropic release leading to the preovulatory surge of Luteinizing Hormone (LH), an obligatory event for reproduction to occur, might be ultimately regulated. We have used two animal models for these studies: hens, showing daily LH preovulatory surges for 15-25 days and ewes, showing an LH preovulatory surge at the end of each follicular phase in a 16-day estrous cycle. In our studies, we have concentrated in assessing the hypothalamic control of LHRH by three of its ME putative inputs: -endorphin (END), dopamine (DA), and neuropeptide Y (NPY). Through our immunocytochemical studies we mapped the hypothalamic distribution of LHRH and these three ME putative inputs, in hens and ewes. On the other hand, in our release studies, we used in vitro incubations of ME tissue and push-pull cannula (PPC) sampling of in vivo neuropeptide release from discrete areas of the ME. In addition to our release studies, we assessed the possibility that enzymatic degradation of LHRH in the ME might be a regulated event of some physiological significance. Furthermore, we recently determined steady-state levels of mRNA and expression of c-fos antigens, in some of these putative inputs to ME-LHRH neuronal terminals. In addition, we have developed an extremely sensitive capillary electrophoresis-based assay, that uses fluorescence microscopy detection of derivatized samples, for the determination of multiple neuropeptides in ME-PPC perfusate samples.

Specific Projects

Effect of intragastric ethanol on the neuroendocrine control of the preovulatory release of LHRH by endorphin and neuropeptide Y at the hypothalamic median eminence.?

Special Techniques

In vivo neuropeptide release from discrete brain areas in stereotaxically instrumented ewes undergoing a synchronized follicular phase of their estrous cycle.


Title Location Year
Professor Rutgers University 1993
Assistant Professor Rutgers University 1982
Assistant Professor Austral University, Medical School, Chile 1972


Degree Location Year
Ph.D. University of Texas, Dallas 1980
Postdoc University of Pittsburgh & State University of New York 1980
Postdoc University of Texas, Dallas 1977
Postdoc Michigan State University, E. Lansing 1975
DVM Austral University, Vet School, Chile 1972

National Committees

Ad hoc reviewer for grants submitted to USDA

Professional Societies

Endocrine Society
Society for Neuroscience
American Physiological Society

Editorial Positions

Editorial Board Member of The Journal of Capillary Electrophoresis
Ad hoc reviewer for:
Biology of Reproduction


11:067:450 Endocrinology
01:146:450 Endocrinology
16:761:501 Mammalian Physiology
11:067:000 Neuroendocrinology
16:067:612 Recent Advances in Endocrinology
01:140:450B1 General Endocrinology
01:119:356B1 System Physiology

Representative Publications

Boyadjieva N, Advis JP, and Sarkar DK(2000). Ethanol reduces the effect of hypothalamic -endorphin on natural killer cell activity. Annual meeting of the Endocrine Society, Toronto, Canada.

Advis JP, Klein J and Sarkar DK (2000). Effects of ethanol on secretion of hypothalamic neuropeptides regulating preovulatory LH release in sheep. Annual meeting of the Research Society on Alcoholism (RSA).

Buntin JD, Advis JP, Ottinger MA, Lea RW and Sharp PJ (1999). An analysis of physiological mechanisms underlying the antigonadotropic action of intracranial prolactin in Ring Doves. General and Comparative Endocrinology 114, 97-107.

Park S, Hung W-L, Guzman N, and Advis JP (1996). Determination of neuropeptides by capillary electrophoresis. In Methods in Molecular Biology, Vol 73: Neuropeptide Protocols. Chapter 11. Edited by B Irvine and C Williams, Humana Press Inc. p101-111.

Contijoch AM, Malamed S, McDonald JK, and Advis JP (1993). Neuropeptide Y regulation of LHRH release at the median eminence: immunocytochemical and physiological evidence in hens. Neuroendocrinology 57:135-145.

Contijoch AM, Gonzales CD, Malamed S, Sarkar DK, Troncoso S and Advis JP (1993). -endorphin regulation of LHRH release at the median eminence level: immunocytochemical and physiological evidence in hens. Neuroendocrinology 57: 365-373.

Advis JP and Contijoch AM (1993). The median eminence as a site for neuroendocrine control of reproduction in hens. Poultry Science 72: 932-939.?

Advis JP and Guzman NA (1993). Capillary electrophoresis coupled to fluorescence detection for the determination of?in vivo?release of multiple neuropeptides from the ewe median eminence. J. Liq.Chromat.16: 2129-2148.?

Contijoch AM and Advis JP (1993). Median eminence and anterior pituitary degradation of luteinizing hormone releasing hormone in hens undergoing changes in luteinizing hormone secretion. Poultry Sci 72: 1756-1763.

Conover CD, Kuljis RO, Rabii J and Advis JP (1993). Use of a multiple guide cannula assembly and removable push-pull cannula for?in vitro?LHRH release from ewe median eminence. Neuroendocrinology 57: 1119-1132.

Prasad BM, Conover CD, Sarkar DK, Rabii J and Advis JP (1993). Feed restriction in prepubertal lambs: in vivo release of median eminence LHRH, NPY and END and puberty onset. Neuroendocrinology 57: 1171-1181.

Conover CD, Kuljis RO, Sarkar DK, Rabii J and Advis JP (1993). -endorphin regulation of LHRH release in median eminence: immunocytochemical and physiological evidence in ewes. Neuroendocrinology 57: 1182-1195.

Advis JP, Iqbal K, Malick W and Guzman NA (1993). Determinations of multiple neuropeptides in ewe median eminence push-pull cannula perfusates by capillary electrophoresis coupled to fluorescence detection. In Handboock on hormonal assay techniques, edited by F de Pablo, CG Scanes and D Weintraub, Academic Press, Orlando, Florida, Chapter 7, p. 127-144.

Contijoch AM, Gonzales CD, Singh HN, Malamed S, Troncoso S and Advis JP (1992). Dopaminergic regulation of LHRH release at the median eminence level: immunocytochemical and physiological evidence in hens. Neuroendocrinology 55 (3): 290-300.

Contijoch AM, AL Johnson and JP Advis (1990). Norepinephrinestimulated in vitro release of LHRH from median eminence is facilitated by inhibition of LHRHdegrading activity in hens. Biol. Reprod 42: 222-230.

Kuljis, R.O. and J.P. Advis (1989). Immunocytochemical and physiological evidence of a synapse between dopamine and LHRH-containing neurons in the ewe median eminence. Endocrinology 124: 1579-1581.

Advis J.P., C.E. Ahmed, and S.R. Ojeda (1989). Direct hypothalamic control of Vasoactive Intestinal Peptide levels in the developing rat ovary. Brain Research Bulletin 22:605-610.

Advis JP, Hernandez L and Guzman NA (1989). Analysis of brain neuropeptides by capillary electrophoresis: determination of LHRH from ovine hypothalamus. Peptide Research 2 (6): 389-394

Advis JP, Contijoch AM, Urbanski HF and Ojeda SR (1987). TPCK inhibits LHRH-Degrading Activity and increases in vitro LHRH release from the immature rat median eminence. Neuroendocrinology 47: 102-108.

Advis JP, Krause JE and McKelvy JF (1983). Evidence that endopeptidase-catalyzed LHRH contributes to regulation of median eminence LHRH during positive steroid feedback. Endocrinology 112:1147-1149.?

Advis JP and Krause JE (1983). Peptidases in physiological processes involving peptidergic neurons. In Methods in Cellular Neurobiology, Barker and McKelvy, ed, John Wiley, New York, Vol. 2, Chapter 5, pp. 148-184.

Advis JP, Krause JE and McKelvy JF (1982). LHRH peptidase activities in discrete hypothalamic regions and anterior pituitary of the female rat: Apparent regulation during the prepubertal period and first estrous cycle of puberty. Endocrinology 110:1238-1245.

Advis JP, Krause JE and McKelvy JF (1982). LHRH peptidase activities in the female rat: Characterization by an assay based on HPLC. Analytical Biochemistry 125(1):41-49.

Advis JP, Smith-White S and Ojeda SR(1981). Delayed puberty induced by chronic suppression of PRL release in the female rat. Endocrinology 109:1321-1330.

Advis JP, Smith-White S and Ojeda SR (1981). Activation of GH short-loop negative feedback delays puberty in the female rat. Endocrinology 108:1343-1352.

Advis JP, Richards JS and Ojeda SR (1981). Hyperprolactinemia-induced precocious puberty: mechanism(s) by which PRL enhances ovarian P4 responsiveness to gonadotropins. Endocrinology 108:1333-1342.

Advis JP, McCann SM and Negro-Vilar A (1980). Involvement of SCN-mPOA in E2 negative feedback: increments in LHRH and norepinephrine content/turnover after ovx are reversed by E2. Endocrinology 107:892-901

Advis JP, Andrews WW and Ojeda SR (1979). Changes in ovarian steroidal and prostaglandin E responsiveness to gonadotropins during the onset of puberty in the female rat. Endocrinology 104:653-658.

Advis JP, Simpkins J, Chen HT and Meites J(1978). Relation of biogenic amines to onset of puberty in the female rat. Endocrinology 103:11-16.

Advis JP and Ojeda SR (1978). Hyperprolactinemia-induced precocious puberty in female rat: ovarian site of action. Endocrinology 103:924-935.